Three replicate RNA-seq samples were collected at four different time points during Hydractinia symbiolongicarpus embryonic development: at the 4-cell, 16-cell, and 64-cell stages and from 24-hour larvae. Each replicate consisted of pools of embryos or larvae from a single spawning event resulting from a cross of the 291-10 male strain with the 295-10 female strain. RNA-seq libraries were constructed for each sample for a total of 12 libraries. These libraries were then sequenced on the Illumina HiSeq 4000 platform using indexed 75-base paired end reads. Sequence reads were processed and aligned to the H. symbiolongicarpus gene models, counted, and normalized via MedianNorm (Anders and Huber, 2010). Normalized read counts are displayed in the expression plots.
View time-course expression plots of H. symbiolongicarpus developmental gene expression data by entering a gene identifier (e.g., HyS0001.1):